This PhD project aims to advance our understanding of human adenovirus (HAdV) biology and develop next-generation viral vectors for gene therapy. Using our unique collection of more than 40 recombinant HAdV types and advanced viral engineering tools, the project will investigate how adenoviruses interact with blood and endothelial cells to better understand viral persistence, dissemination, and vector safety.
Another objective of this project is to evaluate a directed evolution (ADEVO) platform based on peptide-display libraries to identify fiber-modified adenoviruses with enhanced binding and uptake in target cells. Selected candidates will be converted into replication-deficient vectors and evaluated in primary 2D and 3D cell culture models.
The project combines molecular virology, viral vector engineering, and advanced cell culture systems, contributing to the development of safer and more effective gene therapy approaches.